|
Print Version
|
Safety
|
BioSafety Level II
|
Organism
|
Human
|
Source Organ
|
Fetal Lung Fibroblasts (MRC-5)
|
Picture
|
|
Image
|
|
Concentration
|
106Cells/ml
|
Induction
|
iPS-27 was generated using lentiviral transduction of human fetal lung fibroblasts with cMYC, KLF4, OCT4 and SOX2.
|
Pluripotency
|
Positive for markers Nanog, Tra-1-60, Tra-1-80 and SSEA-3 by immunofluorescence and flow cytometry. Capable of differentiating into derivatives of the 3 germ layers.
|
Propagation
|
The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: serum replacer to a final concentration of 20%; b-FGF to a final concentration of 6ng/ml; 1 mM L-glutamine and 2-mercaptoethanol solution; 1X Non-essential amino acids. Sterilize the media through a 0.22 micron filter before use. This cell line needs to be cultured on mouse embryonic fibroblasts (ABM T2001) feeder cells. Atmosphere:air: 95%, CO2: 5%; Temperature: 37.0°C.
|
Subculturing
|
1. Remove and discard culture medium.
2. Add 2.0mL of Dispase solution to flask and incubate the cells at 37°C to facilitate dispersal. Observe cells under an inverted microscope until cell layer is dispersed.
3.Transfer the dispase solutions containing colonies into a conical tube.
4. Centrifuge cells at 1000rpm for 3 minutes to pellet.
5. Gently aspirate out the solution, leaving pellet undisturbed.
6. Resuspend pellet in fresh culture medium and plate on MEF feeder plates.
7. Incubate cultures at 37°C.
|
Storage
|
-180°C
|
Shipping
|
On Dry Ice
|
Notes
|
For Research Use Only, not for therapeutic or diagnostic purposes.
|
References
|
1. Stuart M Chambers, Christopher A Fasano, Eirni P Papapetrou, Mark Tomishima, Michel Sadelain and Lorenz Studer. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 26, 275-280 (2009).
2. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. Aug 4;106(31):12759-64. (2009).
|
关键字
|
iPS-27 Cells;iPS-27 Cells;iPS-27 Cells;iPS-27 Cells;iPS-27 Cells;iPS-27 Cells
|
公司简介
|
合肥星肽生物科技有限公司作为专业的ABM中国代理商,专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。ABM安徽代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务,对购买项目的前期资料提供,中期合同保证,后期货物跟踪到*终售后的确保项目准确到位,都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得*上等服务!
|
联系方式
|
何经理,合肥星肽生物科技有限公司,Tel:0551-63802898 400-8702-898,
E-mail:info@qingbio.com http://m.haomenmc.com QQ:514713116
|